Molecular Archives

Posted inin Molecular

Gel Electrophoresis of PCR Amplicons: Key Considerations for Accurate Results

Posted byby Franklin Veaux
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2 min

Gel Electrophoresis of PCR Amplicons: Key Considerations Gel electrophoresis of PCR amplicons provides a visual representation of DNA fragment sizes, helping to confirm PCR success and identify primer dimers or...

Posted inin Molecular

Learn Molecular Biology Basics: Practical Steps and Resources for Beginners

Posted byby Franklin Veaux
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8 min

How to Learn Molecular Biology to a Basic Level Learning molecular biology to a basic level requires a strong grasp of foundational biology, practical lab experience, engagement with reputable online...

Posted inin Molecular

Why RNA Primers Are Essential for DNA Replication Instead of DNA Primers

Posted byby Franklin Veaux
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2 min

Why RNA Primer is Used in DNA Replication Instead of DNA Primers? RNA primers are used in DNA replication because DNA polymerases cannot initiate DNA synthesis de novo; they require...

Posted inin Molecular

How to Properly Seed 6-Well Transwell Inserts for Epithelial Cells: A Step-by-Step Guide

Posted byby Franklin Veaux
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2 min

How to Properly Seed 6-Well Transwell Inserts for Epithelial Cells Proper seeding in 6-well transwell inserts requires attention to cell density, media volume, and cell viability factors to ensure optimal...

Posted inin Molecular

How to Measure an Electrophoresis Gel: A Step-by-Step Guide for Accurate Results

Posted byby Franklin Veaux
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5 min

How to Measure an Electrophoresis Gel Measuring an electrophoresis gel involves comparing the migration distance of DNA bands to a known ladder or marker, using consistent reference points, and selecting...

Posted inin Molecular

Producing Common Lab Proteins In-House: Taq Polymerase and More

Posted byby Franklin Veaux
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6 min

Which Proteins Commonly Used as Lab Reagents Can Be Produced “In-House”? Several key proteins used frequently in molecular biology, such as Taq polymerase, TEV protease, and Cas9 nuclease, can be...

Posted inin Molecular

How to Read GST Pulldown Assay Results and Interpret Protein Interactions

Posted byby Franklin Veaux
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3 min

How to Read GST Pulldown Assay Results Reading GST pulldown assay results requires careful attention to experimental design, controls, and figure clarity to determine if proteins are truly interacting. Not...

Posted inin Molecular

Preparing Acrylamide Gels for Western Blots: Safety, Steps, and Key Tips

Posted byby Franklin Veaux
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2 min

How to Prepare Acrylamide Gels for Western Blots Preparing acrylamide gels for Western blots involves careful handling of toxic reagents and precise mixing of components. Acrylamide polymerizes to form a...

Posted inin Molecular

Centrifuging Frozen Blood Samples: Assessing Value Loss and Best Practices

Posted byby Franklin Veaux
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3 min

Centrifuging Blood Sample After Freezing: Impact on Sample Value Freezing whole blood before centrifugation generally causes loss of sample value, primarily through red blood cell (RBC) damage and hemolysis. This...

Posted inin Molecular

Troubleshooting Cloning: Key Steps for Successful DNA Transformation and Common Issues Explained

Posted byby Franklin Veaux
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7 min

Troubleshooting Cloning: Ensuring Successful DNA Transformation Troubleshooting cloning primarily involves optimizing competent cell quality, transformation protocols, and plasmid integrity. Addressing each element systematically can identify issues and improve colony yields...