Molecular

Molecular Biology Techniques: Mastering Precision in the Lab Molecular biology techniques demand accuracy, especially in tasks like gel electrophoresis where proper loading affects results. Stress and hand shaking can challenge...

Q5 or Phusion: Choosing the Right DNA Polymerase Both Q5 and Phusion DNA polymerases offer excellent fidelity and performance, with minor differences that influence their use in specific PCR applications....

Designing Primers for Gibson Insert: Why Use Annealing Temperature of Insert Section Only? The annealing temperature for Gibson assembly primers is calculated based solely on the insert-complementary region of the...

Understanding Reading Frames and Open Reading Frames (ORFs) An open reading frame (ORF) is a segment of DNA or RNA that begins with a start codon and extends without interruption...

PolyA Sequence vs SV40 Termination Signal The PolyA sequence and the SV40 termination signal serve distinct roles in gene expression constructs: the directly encoded PolyA sequence protects the upstream transcript...

DNA Shearing by Vortex: Effects and Considerations DNA shearing by vortexing breaks DNA strands into smaller fragments. This fragmentation is generally undesirable for applications like nanopore sequencing, which require long...