Molecular

Understanding Transformation of E. coli: A Detailed Explanation Transformation is the process of introducing foreign DNA, such as plasmids, into Escherichia coli (E. coli) cells to enable new genetic traits....

Help with Blue/White Colony Screening Blue/white colony screening is a molecular biology technique used to distinguish recombinant bacterial colonies from non-recombinant ones based on their color. Effective screening depends on...

Forgot to Add Ethidium Bromide in Gel Electrophoresis: What to Do When Ethidium Bromide (EtBr) is omitted during gel preparation, the gel will run normally but DNA bands will not...

Heat Shock in a Thermocycler Heat shock in a thermocycler is feasible and effective for transforming competent cells, especially E. coli. Setting the thermocycler to a constant 42°C and timing...

HEK293 Cell Culture and CRISPR Cas9 Gene Editing HEK293 cells serve as a valuable model for CRISPR Cas9 gene editing due to their relatively stable karyotype and ease of culture,...

Biology vs Molecular Biology Degree: Understanding the Differences and Implications The key distinction between a Biology degree and a Molecular Biology degree lies in the scale and depth of study....