Molecular Archives

Posted inin Molecular

What is an Open Reading Frame and How Does it Differ from a Reading Frame?

Posted byby Franklin Veaux
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5 min

Understanding Reading Frames and Open Reading Frames (ORFs) An open reading frame (ORF) is a segment of DNA or RNA that begins with a start codon and extends without interruption...

Posted inin Molecular

PolyA Sequence and SV40 Termination Signal: Key Differences and Implications for Gene Studies

Posted byby Franklin Veaux
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2 min

PolyA Sequence vs SV40 Termination Signal The PolyA sequence and the SV40 termination signal serve distinct roles in gene expression constructs: the directly encoded PolyA sequence protects the upstream transcript...

Posted inin Molecular

DNA Shearing by Vortex: Impact on DNA Quality and Strategies for Nanopore Sequencing

Posted byby Franklin Veaux
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5 min

DNA Shearing by Vortex: Effects and Considerations DNA shearing by vortexing breaks DNA strands into smaller fragments. This fragmentation is generally undesirable for applications like nanopore sequencing, which require long...

Posted inin Molecular

ELI5: Understanding In Situ Hybridization Probes and Their Applications

Posted byby Franklin Veaux
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2 min

ELI5: What Are In Situ Hybridization Probes? In situ hybridization probes are specially made RNA strands designed to find and attach to matching RNA sequences inside cells or tissues, allowing...

Posted inin Molecular

Cloning and Subcloning: Concepts, Techniques, and Impact in Biomedical Research

Posted byby Franklin Veaux
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8 min

Cloning and Subcloning: Defining Concepts and Techniques Cloning is the process of generating an exact genetic copy of a biological entity, such as a cell, DNA segment, or organism. Subcloning,...

Posted inin Molecular

Career Guidance for Microbiology Graduates Facing Job Challenges

Posted byby Franklin Veaux
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5 min

Seeking Career Advice: Microbiology Graduate Struggling with Job Opportunities Microbiology graduates can access various career paths, especially within pharma, clinical labs, and healthcare sectors. This article highlights practical job opportunities,...

Posted inin Molecular

Best Method for Purifying DNA Vector After Digesting: Strategies and Insights

Posted byby Franklin Veaux
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4 min

Best Way to Purify DNA Vector After a Digest The best way to purify a DNA vector after a digest is to perform gel purification to separate the linearized plasmid...

Posted inin Molecular

Difference Between Epitope and PAMP: Key Definitions and Functional Insights

Posted byby Franklin Veaux
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2 min

Difference Between Epitope and PAMP The key difference between epitope and PAMP lies in their roles in the immune response: a PAMP is a molecular pattern recognized by the innate...

Posted inin Molecular

TaqMan Probe Design Help: Key Points, Tools, Best Practices, and Validated Primers

Posted byby Franklin Veaux
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1 min

TaqMan Probe Design Help TaqMan probe design relies on careful selection of primers and probes to ensure specificity and efficiency in real-time PCR assays. Several reliable tools and resources help...

Posted inin Molecular

DNA Fingerprinting: Understanding Techniques, Genetic Markers, and Historical Approaches

Posted byby Franklin Veaux
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5 min

Understanding DNA Fingerprinting DNA fingerprinting, also called DNA profiling, identifies individuals by analyzing unique patterns in their DNA. It plays a vital role in forensic investigations and paternity testing. The...