Molecular

Western Blot Protocol: Is Blocking Overnight Advisable? Blocking overnight during Western blotting is acceptable but not mandatory; it depends on protein abundance, antibody specificity, and workflow preferences. Typical blocking duration...

How Does ATP Release Energy? ATP releases energy not merely by breaking a phosphate bond, but through the formation of more stable products and the coupling of its hydrolysis to...

Difference Between Using Diluted Water and Buffers as Blank in UV Spectrophotometry In UV spectrophotometry, the choice between diluted water and buffers as a blank depends on the solvent environment...

mRNA IVT Plasmid Template Questions The best method to detect polyA tails in mRNA IVT plasmid templates is Sanger sequencing. This method remains the most common and reliable technique for...