Molecular Archives

Posted inin Molecular

Accessing “Current Protocols in Cell Biology” and Elsevier Papers: Key Methods and Considerations

Posted byby Franklin Veaux
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2 min

Access to “Current Protocols in Cell Biology” (Wiley) and Elsevier Papers Access to “Current Protocols in Cell Biology” (Wiley) and Elsevier papers depends primarily on institutional subscriptions, individual purchases, or...

Posted inin Molecular

PhD or Master’s? A Guide to Choosing the Right Graduate Path in Science

Posted byby Franklin Veaux
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4 min

PhD or Master’s? Choosing the Right Path in Science The choice between pursuing a PhD or a Master’s degree depends primarily on one’s career goals, financial considerations, and the job...

Posted inin Molecular

ThermoFisher SYBR Green Real-Time PCR Issues: Understanding Ct Value Variations

Posted byby Franklin Veaux
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4 min

ThermoFisher SYBR Green Real-Time PCR Problem Comparing cycle threshold (Ct) values between SYBR Green and TaqMan assays often leads to confusion due to inherent differences in chemistry and detection methods....

Posted inin Molecular

Quickest Way to Learn Molecular Biology: Strategies and Tips for Effective Study

Posted byby Franklin Veaux
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5 min

Quickest Way to Learn Molecular Biology The quickest way to learn molecular biology combines focused textbook study, hands-on lab experience, and targeted online resources. This approach balances theory with practice,...

Posted inin Molecular

T4 DNA Ligase Expired for Four Years: Will It Still Be Effective?

Posted byby Franklin Veaux
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5 min

T4 DNA Ligase Expired 4 Years Ago: Any Chance It Would Still Work? Yes, there is a chance that T4 DNA ligase expired 4 years ago may still work, but...

Posted inin Molecular

Site-Directed Mutagenesis: Key Insights on PCR Settings and Effective Primer Design

Posted byby Franklin Veaux
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5 min

Site-Directed Mutagenesis: PCR Settings and Primer Design Site-directed mutagenesis requires precise PCR settings and carefully designed primers to ensure successful mutation incorporation. The process relies heavily on primer length, composition,...

Posted inin Molecular

Questions Regarding mRNA Translation: Key Concepts, Requirements, and Processes

Posted byby Franklin Veaux
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2 min

Questions Regarding mRNA Translation mRNA translation requires a specific mRNA structure and regulatory proteins to initiate and proceed. Translation begins only at the correct start site on the mRNA, controlled...

Posted inin Molecular

Pelagibacter ubique: Understanding Its Abundance and Key Molecular Mechanisms

Posted byby Franklin Veaux
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3 min

Why Pelagibacter ubique is the Most Abundant Bacteria Pelagibacter ubique is the most abundant bacteria because it combines extreme cellular and genomic streamlining with highly efficient nutrient uptake and metabolic...

Posted inin Molecular

Impact of Resuspending DNA Pellet in 10X TE Buffer: Will Your DNA Be Affected?

Posted byby Franklin Veaux
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1 min

Impact of Resuspending DNA Pellet in 10X TE Buffer Resuspending a DNA pellet in 10X TE buffer generally does not damage the DNA, but it can affect downstream applications. The...

Posted inin Molecular

Is Unwrapped DNA Stable? Examining Chemical Stability and the Role of Histones

Posted byby Franklin Veaux
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2 min

Is DNA – on its own, not wrapped around histones – stable? DNA remains chemically stable even when not wrapped around histones, although some DNA conformations rely on histones for...