Molecular Archives

Posted inin Molecular

Understanding Specific Activity in Enzymes: Definition, Measurement, and Significance

Posted byby Franklin Veaux
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2 min

Specific Activity in Enzymes: Definition and Importance Specific activity is a measure of enzyme purity expressed as the amount of product formed per unit time per milligram of total protein....

Posted inin Molecular

Why Are Introns Longer Than Exons? Key Factors and Insights on Their Length Variation

Posted byby Franklin Veaux
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4 min

Why Are Introns Longer Than Exons? Introns are generally longer than exons due to a combination of evolutionary, genomic, and functional factors, especially in mammals. This pattern arises largely because...

Posted inin Molecular

Can Smearing in PCR Be Removed? Insights on Cleanup Protocols and Result Enhancement

Posted byby Franklin Veaux
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4 min

Can Smearing or Streakiness Be Removed by PCR Cleanup? Examining Cleanup Protocols to Enhance PCR Results Direct PCR cleanup alone often cannot fully remove smearing or streakiness from PCR products....

Posted inin Molecular

Top Software for Generating Primers for Gibson Assembly: A Comprehensive Guide

Posted byby Franklin Veaux
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5 min

Software for Generating Primers for Gibson Assembly Multiple software tools assist in generating primers for Gibson assembly, including NEBuilder, SnapGene, and online tools like NEB’s web utility. These tools streamline...

Posted inin Molecular

How DNA Base Excision Repair (BER) Differs from Mismatch Repair (MMR) Systems

Posted byby Franklin Veaux
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2 min

Differences Between DNA Base Excision Repair (BER) and Mismatch Repair (MMR) Systems DNA Base Excision Repair (BER) and Mismatch Repair (MMR) differ in their timing, targets, and mechanisms in maintaining...

Posted inin Molecular

Optimize Your DIY NGS (KAPA) Quantification with Enhanced qPCR Efficiency Techniques

Posted byby Franklin Veaux
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2 min

DIY NGS (KAPA) Quantification – Optimizing qPCR Efficiency DIY NGS (KAPA) quantification can achieve reliable qPCR efficiency by refining standards, optimizing qPCR protocols, and addressing pipetting accuracy. Careful attention to...

Posted inin Molecular

Question Regarding Proteinase K and Lysis Buffer in DNA Isolation Process

Posted byby Franklin Veaux
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3 min

Understanding the Roles of Proteinase K and Lysis Buffer in DNA Isolation Proteinase K and Lysis Buffer work together to efficiently isolate high-quality DNA by breaking down cellular components and...

Posted inin Molecular

Knowledge of Chemistry Essential for Molecular Biologists: Core Concepts and Applications

Posted byby Franklin Veaux
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2 min

What Knowledge of Chemistry Would a Molecular Biologist Need to Know? Molecular biologists need a strong foundation in biochemistry supported by general and organic chemistry knowledge to grasp molecular interactions...

Posted inin Molecular

Top Laptops for PhD Students: Key Features and Recommendations

Posted byby Franklin Veaux
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3 min

Best Laptop for PhD Students: Key Recommendations The best laptop for a PhD depends on your preferred operating system, workload requirements, and research needs. For Windows users, Lenovo ThinkPads with...

Posted inin Molecular

Understanding Acceptable 260/280 Readings with Low 260/230 Ratios and Their Impacts

Posted byby Franklin Veaux
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2 min

Understanding Acceptable 260/280 But Low 260/230 Readings Acceptable 260/280 readings paired with low 260/230 ratios often indicate contamination by substances such as chaotropic agents or residual salts, which interfere with...