Molecular

How to Denature DNA Irreversibly Without Reannealing Irreversible DNA denaturation without reannealing is achievable by combining chemical and physical treatments that disrupt hydrogen bonding and prevent strands from reassociating. This...

Help with Electrophoresis Troubleshooting Troubleshooting electrophoresis involves systematic checks of ladder integrity, buffer and gel preparation, staining and visualization, and running conditions. This approach ensures clear DNA or protein separation...

RNA Extraction from Fixed Tissue: Understanding Low 260/230 with Good 260/280 Ratios When extracting RNA from formalin-fixed, paraffin-embedded (FFPE) tissue, a good 260/280 ratio combined with a low 260/230 ratio...

Free Software for Sequencing Assembly Several free tools assist with sequencing assembly, each with unique features suitable for different needs. These software packages aid in aligning, assembling, or verifying sequencing...

How Can I Tell If My GFP Is Expressing? To determine if Green Fluorescent Protein (GFP) is expressing, the primary approach is to check for fluorescence under a microscope equipped...

Pitt Honors vs Penn State Honors for Molecular Biology Pitt and Penn State Honors programs both provide strong molecular biology education, but they differ in research funding, undergraduate experience opportunities,...