Molecular

Designing Primers for Gibson Insert: Why Use Annealing Temperature of Insert Section Only? The annealing temperature for Gibson assembly primers is calculated based solely on the insert-complementary region of the...

Difference Between Denaturing and Native Gels Denaturing gels contain chemicals that disrupt the secondary structure of nucleic acids, allowing their separation based solely on size, while native gels preserve the...

Understanding Primer Design for PCR Primer design for PCR requires creating two primers of about 20 base pairs each: a forward primer copied from the 5’ end of the target...

DNA Shearing by Vortex: Effects and Considerations DNA shearing by vortexing breaks DNA strands into smaller fragments. This fragmentation is generally undesirable for applications like nanopore sequencing, which require long...

ELI5: What Are In Situ Hybridization Probes? In situ hybridization probes are specially made RNA strands designed to find and attach to matching RNA sequences inside cells or tissues, allowing...

Cloning and Subcloning: Defining Concepts and Techniques Cloning is the process of generating an exact genetic copy of a biological entity, such as a cell, DNA segment, or organism. Subcloning,...