Molecular Archives

Posted inin Molecular

How to Check RNA Quality: Methods for Purification, Integrity Assessment, and Data Interpretation

Posted byby Franklin Veaux
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2 min

How to Check the RNA Quality? Checking RNA quality involves assessing RNA integrity after extraction, typically using purification followed by visualization methods such as gel electrophoresis or advanced instruments like...

Posted inin Molecular

Blunt End Ligation: Overview, Challenges, Alternatives and Strategies for Success

Posted byby Franklin Veaux
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7 min

Blunt End Ligation: Overview, Challenges, and Alternatives Blunt end ligation is a molecular cloning technique that joins DNA fragments without overhanging nucleotides, but it is generally inefficient compared to sticky...

Posted inin Molecular

Key Insights on Western Blot: Understanding Its Purpose, Limitations, and Comparison to RNA-seq

Posted byby Franklin Veaux
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4 min

Understanding the Western Blot: Answering Key Questions A Western blot is a technique used to detect specific proteins in a sample, measuring both their abundance and molecular weight. It is...

Posted inin Molecular

Effective Strategies to Study Cell Biology and Master Key Concepts

Posted byby Franklin Veaux
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2 min

How to Actually Study Cell Biology? To effectively study cell biology, focus on understanding concepts and relationships rather than just memorizing facts. Build a strong conceptual framework, use analogies, interact...

Posted inin Molecular

Where to Find Molecular Biology of the Cell 7th Edition: Accessing, Highlights, and Key Points

Posted byby Franklin Veaux
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2 min

Where to Find Molecular Biology of the Cell 7th Edition The 7th edition of Molecular Biology of the Cell by Alberts is available online as a digital copy through the...

Posted inin Molecular

Can You Secure Paid International Internships? Insights and Opportunities

Posted byby Franklin Veaux
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7 min

Is It Possible to Get Paid International Internships? Yes, it is possible to get paid international internships, but the opportunities are limited, competitive, and often dependent on structured programs, industry...

Posted inin Molecular

Troubleshooting PCR Primer Issues: Key Insights and Solutions for Effective Results

Posted byby Franklin Veaux
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2 min

Understanding and Troubleshooting Primer Issues in PCR Primer-related problems frequently cause PCR failures and misinterpretation of results. Common issues include primer dimers, incorrect primer concentrations, and suboptimal primer design or...

Posted inin Molecular

SuperScript VILO vs SuperScript II: A Comparison for First Strand Synthesis Performance

Posted byby Franklin Veaux
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1 min

SuperScript VILO vs SuperScript II for First Strand Synthesis SuperScript VILO offers a significantly improved performance over SuperScript II in first strand cDNA synthesis. Its enhanced efficiency makes it the...

Posted inin Molecular

PCR Inhibition: Factors Affecting DNA Quantity and Magnesium Ion Interactions

Posted byby Franklin Veaux
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2 min

Understanding PCR and Inhibition Polymerase Chain Reaction (PCR) can be inhibited by multiple factors, with DNA quantity and magnesium ion availability playing key roles. Excess DNA may reduce PCR efficiency,...

Posted inin Molecular

Advice on CRISPR/Cas: Key Recommendations and Practical Strategies for Gene Editing Success

Posted byby Franklin Veaux
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7 min

Advice on CRISPR/Cas Systems The CRISPR/Cas technique benefits from careful choice of delivery system, screening methods, and cell line preparation to maximize precision and success. This article explores practical guidance...