Cost of HPLC Assay/Analysis
The cost of an HPLC assay or analysis varies widely depending on several factors including the analyte, sample volume, method development needs, and regulatory requirements.
Factors Impacting HPLC Assay Costs
- Analyte and Number of Samples: The type of analyte greatly influences price. Routine small-molecule analyses often cost a few hundred Euros per sample. Complex analyses, such as those for dioxins with GC/HRMS, can reach thousands per sample, and HPLC costs scale similarly.
- Volume of Samples: Larger batch analyses tend to reduce the cost per sample due to efficiency in processing.
Routine HPLC Assays
When a laboratory routinely performs the assay, costs remain lower. Such assays usually range in the low hundreds of dollars per sample. They benefit from standardized protocols and familiar instrumentation, enabling quicker turnaround and less resource consumption.
New Method Development
Developing a new HPLC method requires additional expenses for consumables like columns and reagents. Time invested by analytical scientists and validation steps also add to costs. Development can run from several thousand to tens of thousands of dollars, especially if the method supports new formulations or novel analytes.
GMP vs Non-GMP Costs
| Assay Type | Typical Cost per Sample |
|---|---|
| Non-GMP Assay | Approximately $150 – $250 |
| GMP Assay | About double non-GMP costs, plus USP standard fees |
GMP assays require strict adherence to regulatory standards, staff qualifications, and documentation, raising costs substantially compared to non-GMP analyses.
Engaging Contract Analytical Companies
Outsourcing to contract analytical firms can be an efficient approach. These companies offer expertise and validated methods, which often speeds up assay delivery and provides reliable quality control at competitive prices.
Key Takeaways
- HPLC assay costs depend heavily on analyte, sample number, and method complexity.
- Routine assays cost in the low hundreds per sample; new method development significantly increases expenses.
- GMP assays cost approximately twice as much as non-GMP ones, due to compliance requirements.
- Contract analytical companies provide specialized services that may optimize cost and efficiency.
What factors influence the cost of an HPLC assay?
The cost depends on the analyte being tested and the number of samples. Some tests cost only a few euros per sample, while more complex analyses can be much higher.
How much does a routine HPLC assay typically cost?
Routine assays usually cost in the low hundreds of dollars or euros per sample. Labs running the test regularly can process samples faster, keeping costs down.
Why is new method development for HPLC expensive?
Developing a new HPLC method requires time, reagents, and columns, which can cost thousands. Creating methods for new formulations or analytes can run into tens of thousands.
What is the cost difference between GMP and non-GMP HPLC assays?
Non-GMP assays usually cost between $150 and $250. GMP assays generally cost about twice as much and may include additional expenses for USP standards.
Is it necessary to use a contract analytical company for HPLC assays?
Yes, especially for complex or regulated testing. Contract labs have the needed equipment and expertise to perform assays efficiently and reliably.
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