Kingfisher Apex DNA Binding Beads in Elution Plate
Kingfisher Apex DNA binding beads may appear in the elution plate during plasma DNA extractions, but this presence is usually minimal and manageable. These beads are specifically used for plasma samples and not saliva.
Usage Context
- Beads target plasma DNA extraction exclusively.
- Saliva samples do not utilize these beads in the extraction process.
Bead Presence and Management
Some beads occasionally carry over into the elution plate. The number is typically small and rarely problematic.
If bead contamination in the elution volume is a concern, placing a magnet beneath the elution plate effectively reduces bead presence in the final elution tube. This simple technique attracts residual beads, improving sample purity.
Technique and Experience Influence
- New users experience more frequent bead carryover than experienced operators.
- Vigorously mixing the beads before adding them to the plate ensures uniform bead distribution and reduces bead leftovers post-elution.
This suggests that operator skill strongly impacts the cleanliness of the elution, though proper training and handling can alleviate bead contamination.
Effect on Downstream Applications
Initial extractions with some bead carryover do not compromise sequencing quality. Controls processed by less experienced technicians yielded sequencing results comparable to those from veteran staff. Thus, minor bead presence appears inconsequential for sequencing performance in plasma DNA extraction.
Summary of Key Points
- Kingfisher Apex beads are designed for plasma DNA, not saliva.
- Beads can occasionally be present in the elution plate but usually in small amounts.
- Using a magnet under the elution plate helps eliminate bead carryover.
- Experience and thorough bead mixing reduce residual beads effectively.
- Minor bead presence does not affect sequencing results significantly.
Q: Can Kingfisher Apex DNA binding beads be used for saliva samples?
No. These beads are used specifically for plasma extractions and not for saliva samples.
Q: Is it normal to find beads in the elution plate after extraction?
Yes, finding a small number of beads in the elution plate is common. Usually, the quantity is minimal and does not affect results.
Q: How can I prevent beads from contaminating my final elution?
Using a magnet under the elution plate helps remove beads from the final elution tube effectively.
Q: Does experience reduce bead carryover in elution plates?
Yes, bead carryover is more frequent during initial uses but decreases with experience and better technique.
Q: Does vigorous mixing of beads before use affect bead carryover?
Mixing beads thoroughly before adding them to the plate improves consistency and reduces leftover beads in the elution plate.
Q: Do beads in the elution plate affect sequencing quality?
Despite some bead presence in early extractions, sequencing results remained consistent and comparable to those by experienced technicians.
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