Molecular

SuperScript VILO vs SuperScript II for First Strand Synthesis SuperScript VILO offers a significantly improved performance over SuperScript II in first strand cDNA synthesis. Its enhanced efficiency makes it the...

Understanding PCR and Inhibition Polymerase Chain Reaction (PCR) can be inhibited by multiple factors, with DNA quantity and magnesium ion availability playing key roles. Excess DNA may reduce PCR efficiency,...

Advice on CRISPR/Cas Systems The CRISPR/Cas technique benefits from careful choice of delivery system, screening methods, and cell line preparation to maximize precision and success. This article explores practical guidance...

Protein Analysis, Translation, and Reverse Complement Tools Protein analysis, translation, and reverse complement tools enable researchers to manage, analyze, and interpret genetic sequences efficiently. These tools streamline workflows from sequence...

An Ideal Temperature for RT-qPCR Cycles The ideal temperature for RT-qPCR cycles varies mainly by the polymerase used. Generally, denaturation occurs at 95°C, annealing near 60°C, and extension at 60–72°C...

Why is This RNA Oligo Making a Weird Donut Shape in My Urea Gel? The donut shape in the RNA oligo band on a urea gel usually results from overloading...