Molecular

Help Understanding Primer Design Primer design involves creating two short DNA sequences that bind specifically to target regions on a DNA template, allowing selective amplification of the desired sequence. This...

Molecular Biology Techniques Molecular biology techniques encompass various methods used to study and manipulate genetic material, including molecular cloning, gene manipulation, and interdisciplinary approaches integrating biophysics. Molecular Cloning Molecular cloning...

16S rRNA PCR Help: Key Considerations and Troubleshooting Effective 16S rRNA PCR depends on precise primer design, correct target gene selection, sample quality, and validating amplification through controls. Issues in...

Understanding Insertion Mutation An insertion mutation is a type of genetic mutation where extra nucleotides are added into a DNA sequence. This addition can disrupt the normal reading frame of...

Screwed up DNA extraction – too diluted. Halp! If your DNA sample is too diluted after extraction, the quickest way to concentrate it is ethanol precipitation. This method uses cold...

What Is Wrong with My PCR? When PCR results are unclear or unexpected, several factors related to the procedure and reagents might be causing the problem. These include electrophoresis settings,...