Molecular Archives

Posted inin Molecular

Understanding the Central Dogma: Key Concepts and Misinterpretations in Molecular Biology

Posted byby Franklin Veaux
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6 min

Understanding the Central Dogma of Molecular Biology The central dogma describes the flow of genetic information from DNA to RNA to proteins, highlighting a unidirectional transfer essential for protein synthesis,...

Posted inin Molecular

Career Paths After Three Years as a Biotech Research Associate in India

Posted byby Franklin Veaux
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2 min

Career Options After Three Years as a Research Associate in Indian Biotech Where to go after three years as a Research Associate making ₹30,000 per month depends on goals, skill...

Posted inin Molecular

Methods to Denature DNA Irreversibly Without Reannealing

Posted byby Franklin Veaux
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2 min

How to Denature DNA Irreversibly Without Reannealing Irreversible DNA denaturation without reannealing is achievable by combining chemical and physical treatments that disrupt hydrogen bonding and prevent strands from reassociating. This...

Posted inin Molecular

Help with Electrophoresis Troubleshooting: Common Issues and Solutions in Gel Electrophoresis

Posted byby Franklin Veaux
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4 min

Help with Electrophoresis Troubleshooting Troubleshooting electrophoresis involves systematic checks of ladder integrity, buffer and gel preparation, staining and visualization, and running conditions. This approach ensures clear DNA or protein separation...

Posted inin Molecular

RNA Extraction from Fixed Tissue: Good 260/280 but Low 260/230 Ratios Explained

Posted byby Franklin Veaux
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2 min

RNA Extraction from Fixed Tissue: Understanding Low 260/230 with Good 260/280 Ratios When extracting RNA from formalin-fixed, paraffin-embedded (FFPE) tissue, a good 260/280 ratio combined with a low 260/230 ratio...

Posted inin Molecular

Modelling a Cell Membrane: Key Concepts and Tools for Lipid Bilayer Analysis

Posted byby Franklin Veaux
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2 min

Modelling a Cell Membrane Modelling a cell membrane involves representing its fundamental lipid bilayer structure using various approaches such as lipid nanodiscs, spherical liposomes, or planar bilayers. These models help...

Posted inin Molecular

DNA Sequencing in Agarose Gel Electrophoresis: Key Concepts and Challenges

Posted byby Franklin Veaux
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2 min

DNA Sequencing in Agarose Gel Electrophoresis DNA sequencing in agarose gel electrophoresis involves using gel-based separation of DNA fragments to analyze plasmid DNA conformations and assess digestion patterns prior to...

Posted inin Molecular

Skills to Develop as a Molecular Biologist: Essential Techniques and Competencies for Success

Posted byby Franklin Veaux
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2 min

Skills to Develop as a Molecular Biologist Mastering gene editing techniques, cloning methods, bioinformatics, mathematical skills, and soft skills form the foundation for a successful molecular biologist. These competencies ensure...

Posted inin Molecular

Fixed PCR Annealing Temperature at 53°C: Challenges and Recommendations for Improved DNA Yield

Posted byby Franklin Veaux
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3 min

Fixed Annealing Temperature at 53°C for PCR: An Evaluation Using a fixed annealing temperature of 53°C for all primers, regardless of their specific melting temperatures (Tm), is generally not recommended...

Posted inin Molecular

Free Software Options for Sequencing Assembly: Top Tools and Key Features

Posted byby Franklin Veaux
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2 min

Free Software for Sequencing Assembly Several free tools assist with sequencing assembly, each with unique features suitable for different needs. These software packages aid in aligning, assembling, or verifying sequencing...