Molecular Archives

Posted inin Molecular

What’s the Maximum PCR Amplification Length for DNA Fragments?

Posted byby Franklin Veaux
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2 min

What’s the Biggest Fragment PCR Can Amplify? Polymerase chain reaction (PCR) can amplify DNA fragments up to approximately 16.5 kb under optimized conditions, with routine amplification of fragments between 5...

Posted inin Molecular

Salaries in Genetics and Molecular Biology: A Comparative Overview by Country

Posted byby Franklin Veaux
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2 min

Salaries in Genetics and Molecular Biology Across Different Countries Salaries in genetics and molecular biology vary significantly depending on the country. In Germany, an annual income typically ranges from 40,000...

Posted inin Molecular

What Happens If SV40 Promoter Enters Your Cells? Impacts on Gene Expression and Genetic Stability

Posted byby Franklin Veaux
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4 min

What Happens If SV40 Promoter Enters Your Cell? If an SV40 promoter enters a cell, it may initiate transcription of downstream RNA temporarily but does not integrate into the host...

Posted inin Molecular

Why eDNA Professionals Show Caution Over Contamination Risks and Protocols

Posted byby Franklin Veaux
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5 min

Why Are All eDNA Folks Paranoid? Environmental DNA (eDNA) researchers tend to exhibit high levels of caution due to the pervasive risk of contamination, the sensitivity of detection methods, and...

Posted inin Molecular

GFP: Characteristics, Applications, and Importance in Protein Visualization

Posted byby Franklin Veaux
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2 min

Understanding GFP (Green Fluorescent Protein) GFP is a protein that glows brightly when exposed to specific light, allowing visualization of proteins inside living cells. This glowing property makes GFP an...

Posted inin Molecular

Calculate the Net Charge of a Protein: A Step-by-Step Guide

Posted byby Franklin Veaux
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2 min

How to Find the Net Charge Over an Entire Protein Finding the net charge over an entire protein involves obtaining its amino acid sequence, calculating the charge of each residue...

Posted inin Molecular

Plasmid Mapping with Restriction Enzymes: A Guide to Solving Fragment Size Challenges

Posted byby Franklin Veaux
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5 min

Plasmid Mapping with Restriction Enzymes: Problem Solving Guide Plasmid mapping restriction enzyme problems require combining single and double digest fragment data to build a consistent map of restriction sites on...

Posted inin Molecular

4M KCl Preparation: Understanding Colored Precipitate Formation and Solutions

Posted byby Franklin Veaux
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2 min

Preparing 4M KCl Solution and Dealing with Colored Precipitate Preparing a 4M KCl solution often results in colored precipitate due to saturation near its solubility limit, potentially compounded by impurities...

Posted inin Molecular

Function of this Section of a Protein: Roles and Approaches for CASP8AP2

Posted byby Franklin Veaux
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2 min

Function of This Section of a Protein The function of a specific protein section cannot be definitively determined solely by looking at its structure. However, combining structural analysis, sequence information,...

Posted inin Molecular

BCA Assay Question: Key Points for Accurate Protein Quantification and Testing Methods

Posted byby Franklin Veaux
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2 min

BCA Assay Question: Key Considerations in Protein Quantification The BCA assay is a reliable method for protein quantification, but factors such as plate variability and standard curve usage can affect...