Molecular Archives

Posted inin Molecular

Is It Safe to Eat Agarose Gel? Understanding Risks and Health Recommendations

Posted byby Franklin Veaux
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2 min

Is it okay to eat agarose gel? Eating agarose gel from laboratory settings is not safe and should be avoided. Although agarose itself is a natural substance extracted from sea...

Posted inin Molecular

Polycistronic Bacterial Plasmids as T2A Analog: Design, Implementation, and Practical Insights

Posted byby Franklin Veaux
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3 min

Polycistronic Bacterial Plasmid as an Analog of T2A A polycistronic bacterial plasmid functions as an analog to the T2A system by enabling coexpression of multiple proteins from a single mRNA...

Posted inin Molecular

Interesting Bands on a pRAD1 Mini Prep: Key Points on Agarose Gel and Band Identification

Posted byby Franklin Veaux
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3 min

Interesting Bands on a pRAD1 Mini Prep The distinct bands observed on an agarose gel after a pRAD1 mini prep represent different conformations of plasmid DNA, including nicked, linear, supercoiled,...

Posted inin Molecular

Help with Thermo Fisher: Navigating Purchase, Shipping, and Supply Concerns

Posted byby Franklin Veaux
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2 min

Help with Thermo Fisher: Navigating Purchase and Shipping Challenges Thermo Fisher does not ship most lab products to residential addresses due to safety and regulatory reasons. This policy applies especially...

Posted inin Molecular

Best DNA Extraction Protocols for Poor Quality Animal Tissue: Methods and Practices

Posted byby Franklin Veaux
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2 min

Best DNA Extraction Protocol for Poor Quality Animal Tissue The best DNA extraction protocol for poor quality animal tissue combines effective tissue breakdown with protein digestion, careful sample preparation, and...

Posted inin Molecular

Doubt on CRISPR Allelic Exchange Methods with CHOPCHOP Explained

Posted byby Franklin Veaux
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2 min

CRISPR Allelic Exchange Using CHOPCHOP CHOPCHOP facilitates CRISPR allelic exchange by designing guide RNAs (gRNAs) for precise Knock In edits that introduce specific nucleotide changes, employing homology arms to support...

Posted inin Molecular

Primer Optimization for PCR: Enhance Accuracy and Yield with Universal Annealing Temperature

Posted byby Franklin Veaux
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4 min

Primer Optimization in PCR: Enhancing Accuracy and Efficiency Primer optimization is essential for successful polymerase chain reaction (PCR). It involves selecting and refining primer annealing conditions to maximize specificity, yield,...

Posted inin Molecular

Can Any of You Debunk Possible Off-Planet Biological Life Claims?

Posted byby Franklin Veaux
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4 min

Can Any of You Debunk This? Possible Off-Planet Biological Life Disclosure The claim of off-planet biological life disclosure, as circulated in certain circles, faces serious skepticism grounded in scientific rigor...

Posted inin Molecular

Molecular Biology of the Cell: Textbook Editions, Digital Access, and Key Resources

Posted byby Franklin Veaux
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2 min

Molecular Biology of the Cell: Overview and Textbook Availability “Molecular Biology of the Cell” serves as a fundamental resource in understanding cellular mechanisms at the molecular level. It is widely...

Posted inin Molecular

Are Overhangs in Sticky Ends from Restriction Enzymes Always 5’? Understanding the Differences

Posted byby Franklin Veaux
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5 min

Are the Overhangs in Sticky Ends Produced by Restriction Enzymes Always 5’? No, the overhangs in sticky ends produced by restriction enzymes are not always 5’ overhangs. They can be...