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Is Creating a Fusion of Two Proteins Difficult? Creating a fusion protein is generally not difficult, especially when both proteins involved are globular, self-folding, and do not require special folding...

Luciferase/Luciferin In Vitro Bioluminescence Demonstration Luciferase and luciferin together enable bioluminescence, which can be clearly demonstrated in vitro. The visibility of light depends on the luciferase type, concentrations, and reaction...

First CRISPR Experiment as a Molecular Biology Rookie The first CRISPR experiment for a molecular biology novice involves understanding the CRISPR-Cas9 system’s components, designing synthetic guide RNAs, carefully considering expression...

Understanding the Sanger Method of DNA Sequencing The Sanger method sequences DNA by incorporating both normal nucleotides (dNTPs) and special chain-terminating nucleotides (ddNTPs) into a DNA synthesis reaction, creating fragments...