Recommendations for PCR Machines
Thermo Fisher Scientific and BioRad PCR machines stand out as the most reliable and widely used options in many UK laboratories. Both brands offer durable equipment that rarely breaks down, supporting consistent laboratory performance. However, preferences vary depending on departmental needs, with Eppendorf and Roche also recognized as reliable options.
Brand Reputation and Preferences
- Thermo Fisher Scientific: Mixed opinions exist. Some users question the brand’s overall equipment reputation, while others praise its quick ramp-up times and easy maintenance. Thermo PCR machines are standard in UK labs and are commended for their straightforward quality assurance and cleanliness.
- BioRad: Trusted highly within certain departments, alongside Thermo. BioRad machines share a reputation for reliability and are often found as standard issues in laboratory PCR setups.
- Eppendorf and Roche: Selected by some departments, these brands provide strong alternatives depending on specific laboratory requirements.
- ABI (Applied Biosystems) 9700: The used ABI 9700 model is favored for its lifespan, often outperforming newer models like the ProFlex in durability and quality control. Concerns exist about the lack of quality control measures in new ProFlex units.
Performance and Maintenance
Thermo PCR machines offer fast temperature ramp-up, which can improve experiment turnaround time. Their design allows for efficient QA/QC procedures and easy cleaning. This simplifies maintenance tasks and helps sustain reliable operations over long periods.
Comparative Details of Specific Models
| Model | Key Features | Concerns |
|---|---|---|
| ABI 9700 (Used) | Long-lasting, robust, trusted durability | Limited availability as used equipment |
| ProFlex (New) | Modern features | Lack of quality control, uncertain reliability |
| Thermo Fisher PCR | Quick ramp-up, easy cleaning, reliable | Mixed brand reputation |
| BioRad | Reliable, widely used, solid machines | Fewer noted concerns |
Summary of Key Points
- Thermo Fisher and BioRad machines are prevalent in UK labs, known for reliability and ease of use.
- Used ABI 9700 units often outlast newer models like ProFlex and maintain better quality control.
- Equipment choices depend on department preferences, with Eppendorf and Roche also viable options.
- Newer PCR machines sometimes exhibit quality control shortcomings affecting trust in their performance.
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